Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

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Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

In a recent study published in PLOS ONE, researchers developed a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) system to identify severe acute respiratory syndrome coronavirus ...